Photodynamic Approach for Teratoma-Free Pluripotent Stem Cell Therapy Using CDy1 and Visible Light
نویسندگان
چکیده
منابع مشابه
Photodynamic Approach for Teratoma-Free Pluripotent Stem Cell Therapy Using CDy1 and Visible Light
Pluripotent stem cells (PSC) are promising resources for regeneration therapy, but teratoma formation is one of the critical problems for safe clinical application. After differentiation, the precise detection and subsequent elimination of undifferentiated PSC is essential for teratoma-free stem cell therapy, but a practical procedure is yet to be developed. CDy1, a PSC specific fluorescent pro...
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Successful isolation, derivation and culturing of human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem (hiPSCs) cells in laboratory scale has opened new horizones for cell therapy applications such as tissue engineering and regenerative medicine. However, most of the cell therapy protocols using these unique cells require large number of ...
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Stem-toxic small molecules have been developed to induce selective cell death of pluripotent stem cells (PSCs) to lower the risk of teratoma formation. However, despite their high efficacies, chemical-based approaches may carry unexpected toxicities on specific differentiated cell types. Herein, we took advantage of KillerRed (KR) as a suicide gene, to selectively induce phototoxicity using vis...
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Stem cells continuously turn toward the choices of self-renewing, differentiation, migration, quiescence and finally death [1-3]. The embryonic stem cells (ESCs) and their counterparts induced pluripotent stem cells (iPSCs) go through unlimited self-renewal and pluripotency to differentiate into all cell types from all lineages in the body. Therefore, as a renewable source of cells, ESCs and iP...
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ژورنال
عنوان ژورنال: ACS Central Science
سال: 2016
ISSN: 2374-7943,2374-7951
DOI: 10.1021/acscentsci.6b00099